Comparison of relative rates of BTEX biodegradation using respirometry

被引:20
作者
Goudar, CT [1 ]
Strevett, KA [1 ]
机构
[1] Univ Oklahoma, Sch Civil Engn & Environm Sci, Bioenvironm Engn & Sci Lab, Norman, OK 73019 USA
关键词
biodegradation; BTEX; microbial kinetics; respirometry;
D O I
10.1038/sj.jim.2900553
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biodegradation of BTEX by a microbial consortium isolated from a closed municipal landfill was studied using respirometric techniques. The kinetics of biodegradation were estimated from experimental oxygen uptake data using a nonlinear parameter estimation technique, All of the six compounds were rapidly degraded by the microbial culture and no substrate inhibition was observed at the concentration levels examined (200 mg L-1 as COD). Microbial growth and contaminant degradation were adequately described by the Monod equation. Considerable differences were observed in the rates of BTEX biodegradation as seen from the estimates of the kinetic parameters, A three-fold variation was seen in the values of the maximum specific growth rate, mu(max). The highest value of mu(max) was 0.389 h(-1) for p-xylene while o-xylene was characterized by a mu(max) value of 0.14 h(-1), the lowest observed in this study. The half saturation coefficient, K-s, and the yield coefficient, Y, varied between 1.288-4.681 mg L-1 and 0.272-0.645 mg mg(-1), respectively. Benzene and o-xylene exhibited higher resistance to biodegradation while toluene and p-xylene were rapidly degraded. Ethylbenzene and m-xylene were degraded at intermediate rates, In biodegradation experiments with a multiple substrate matrix, substrate depletion was slower than in single substrate experiments, suggesting an inhibitory nature of substrate interaction.
引用
收藏
页码:11 / 18
页数:8
相关论文
共 33 条
[1]   APPLICATION OF RESPIROMETRIC BIODEGRADABILITY TESTING PROTOCOL TO SLIGHTLY SOLUBLE ORGANIC-COMPOUNDS [J].
AICHINGER, G ;
GRADY, CPL ;
TABAK, HH .
WATER ENVIRONMENT RESEARCH, 1992, 64 (07) :890-900
[2]  
Alvarez P J, 1991, Biodegradation, V2, P43, DOI 10.1007/BF00122424
[3]  
ALVAREZ PJJ, 1995, WATER SCI TECHNOL, V31, P15, DOI 10.1016/0273-1223(95)00151-C
[4]   SUBSTRATE INTERACTIONS OF BENZENE, TOLUENE, AND PARA-XYLENE DURING MICROBIAL-DEGRADATION BY PURE CULTURES AND MIXED CULTURE AQUIFER SLURRIES [J].
ALVAREZ, PJJ ;
VOGEL, TM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (10) :2981-2985
[5]   SUBSTRATE INTERACTIONS DURING AEROBIC BIODEGRADATION OF BENZENE [J].
ARVIN, E ;
JENSEN, BK ;
GUNDERSEN, AT .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (12) :3221-3225
[6]   ISOLATION OF A PSEUDOMONAS-STUTZERI STRAIN THAT DEGRADES ORTHO-XYLENE [J].
BAGGI, G ;
BARBIERI, P ;
GALLI, E ;
TOLLARI, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (09) :2129-2132
[7]   NATURAL ATTENUATION OF AROMATIC-HYDROCARBONS IN A SHALLOW SAND AQUIFER [J].
BARKER, JF ;
PATRICK, GC ;
MAJOR, D .
GROUND WATER MONITORING AND REMEDIATION, 1987, 7 (01) :64-71
[8]   BIODEGRADATION KINETICS OF SUBSTITUTED PHENOLICS - DEMONSTRATION OF A PROTOCOL BASED ON ELECTROLYTIC RESPIROMETRY [J].
BROWN, SC ;
GRADY, CPL ;
TABAK, HH .
WATER RESEARCH, 1990, 24 (07) :853-861
[9]  
CHANG MK, 1993, BIOTECHNOL BIOENG, V41, P1057, DOI 10.1002/bit.260411108
[10]  
COLVIN RJ, 1990, 45 PURD U IND WAST C, P501