Asymmetric reduction of ethyl 2-methyl 3-oxobutanoate by Chlorella

被引：12

作者：

Kuramoto, T

Iwamoto, K

Izumi, M

Kirihata, M

Yoshizako, F

机构：

[1] Univ Osaka Prefecture, Adv Sci & Technol Res Inst, Osaka 5998570, Japan

[2] Osaka Univ, Grad Sch Sci, Dept Chem, Osaka 5600043, Japan

[3] Univ Osaka Prefecture, Coll Agr, Dept Appl Biochem, Osaka 5998531, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 03期

关键词：

Chlorella; asymmetric reduction; ethyl; 2-methyl; 3-oxobutanoate; (2S; 3S)-ethyl; 3-hydroxybutanoate; 3R)-ethyl;

D O I：

10.1271/bbb.63.598

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chorella pyrenoidosa Chick reduced ethyl 2-methyl 3-oxobutanoate to the corresponding alcohols with the diastereomer (anti/syn) ratio of 53/47. The enantiomer excesses of anti-(2S, 3S)- and syn-2S, 3R)-hydhoxy esters were 89 and > 99ee% respectively. C. vulgaris and C regularis afforded predominantly the syn-isomer, contrary to C, pyrenoidosa. The differences in the activity of reducing ethyl 2-methyl 3-oxobutanoate were observed among three strains of Chlorella. Addition of 2% metal salts slightly increased the chemical yield of the hydroxy ester.

引用

页码：598 / 601

页数：4

共 19 条

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