Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species

被引：23

作者：

Islam, Md. Sajedul ^{[2
]}

Lian, Chunlan ^{[1
]}

Geng, Qifang ^{[1
]}

Kameyama, Norikazu ^{[3
]}

Hogetsu, Taizo ^{[2
]}

机构：

[1] Univ Tokyo, Asian Nat Environm Sci Ctr, Nishitokyo, Tokyo 1880002, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[3] Univ Ryukyus, Fac Agr, Okinawa 9030213, Japan

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 04期

基金：

日本学术振兴会;

关键词：

cpSSR; mangrove; maternal lineage; Rhizophoraceae;

D O I：

10.1007/s10592-007-9424-x

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 [野生动植物保护与利用];

摘要：

Chloroplast microsatellite (cpSSR) markers were developed for three ecologically and economically important tree species in the mangrove family, Rhizophoraceae: Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa. Noncoding regions of chloroplast DNA (cpDNA) from each species were separately amplified using universal chloroplast primers. Six, two, and three polymorphic cpSSR loci in B. gymnorrhiza, K. candel, and R. stylosa, respectively, were developed from amplified noncoding cpDNA regions. Characterization of 216, 156, and 253 individuals of B. gymnorrhiza, K. candel, and R. stylosa, respectively, collected from different natural mangrove populations (B. gymnorrhiza, 9; K. candel, 7; R. stylosa, 9) on Iriomote Island in Japan showed that these loci provide cpSSR markers with polymorphisms ranging from two to four alleles per locus and gene diversity between 0.027 and 0.480. These cpSSR markers will be useful for analyzing the maternal lineage distributions and population genetic structures of the three species. Several of these markers may also be useful in similar studies of other mangrove species.

引用

页码：989 / 993

页数：5

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