Phagocytic uptake of fluorescent stealth and non-stealth solid lipid nanoparticles

被引：90

作者：

Bocca, C

Caputo, O

Cavalli, RB

Gabriel, L

Miglietta, A

Gasco, MR

机构：

[1] Univ Turin, Dipartimento Med & Oncol Sperimentale, I-10125 Turin, Italy

[2] Univ Turin, Dipartimento Sci & Tecnol Farm, I-10125 Turin, Italy

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 1998年 / 175卷 / 02期

关键词：

colloidal carriers; macrophages; phagocytosis uptake; stealth solid lipid nanoparticles;

D O I：

10.1016/S0378-5173(98)00282-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Fluorescent non-stealth and stealth solid lipid nanoparticles (SLN) were prepared using rhodamine B base as fluorescent marker. The steric stabilization of the nanoparticles was obtained using two lipid derivatives of monomethylpoly(ethylene)glycol 2000 (PEG 2000) as stealth agents: dipalmitoylphosphatidylethanolamine-PEG 2000 and stearic acid-PEG 2000. Stealth and non-stealth SLN were in the nanometer size range. Phagocytosis was evaluated by incubating SLN with murine macrophages and determining the extent of phagocytic uptake spectrofluorimetrically; stealth SLN inhibited phagocytosis to a greater extent than did non-stealth SLN. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：185 / 193

页数：9

共 22 条

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