Spinach chloroplast ATP-dependent endopeptidase Ti-like protease

被引：2

作者：

Benesova, M ^{[1
]}

Durcova, G ^{[1
]}

Kuzela, S ^{[1
]}

Kutejova, E ^{[1
]}

Psenak, M ^{[1
]}

机构：

[1] SLOVAK ACAD SCI,INST MOLEC BIOL,BRATISLAVA 84251,SLOVAKIA

来源：

PHYTOCHEMISTRY | 1996年 / 41卷 / 01期

关键词：

spinacia oleracea; chenopodiaceae; spinach; chloroplasts; ATP-dependent protease;

D O I：

10.1016/0031-9422(95)00613-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The soluble fraction of spinach chloroplast was used for purification and characterization of an ATP-dependent protease. Purification included Q Sepharose Fast Flow, hydroxylapatite and FPLC Superose 6 column chromatography. The isolated enzyme requires ATP and Mg2+ for stimulation and represents a ubiquitin independent serine protease, containing essential sulphydryl group(s). By using fluorogenic peptides a similarity of chloroplast protease to Escherichia coli Ti protease was observed. The chloroplast protease is immunochemically cross - reactive with the bacterial protease Ti.

引用

页码：65 / 69

页数：5

共 42 条

[1]

BEERS EP, 1992, J BIOL CHEM, V267, P15432

[2] INTRACELLULAR PROTEASES [J].

BOND, JS ;

BUTLER, PE .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :333-364

[3] PROTEASES OF MELILOTUS-ALBA MESOPHYLL PROTOPLASTS .2. GENERAL-PROPERTIES AND EFFECTIVENESS IN DEGRADATION OF CYTOSOLIC AND VACUOLAR ENZYMES [J].

CANUT, H ;

DUPRE, M ;

CARRASCO, A ;

BOUDET, AM .

PLANTA, 1987, 170 (04) :541-549

[4]

CHIN DT, 1988, J BIOL CHEM, V263, P11718

[5] PROTEASES IN ESCHERICHIA-COLI [J].

CHUNG, CH .

SCIENCE, 1993, 262 (5132) :372-374

[6]

COOPAS FC, 1993, EUR J CELL BIOL, V61, P81

[7]

DALLING MJ, 1986, PLANT PROTEOLYTIC EN, V2, P125

[8]

DYCK LV, 1993, J BIOL CHEM, V269, P238

[9] THE MECHANISM AND FUNCTIONS OF ATP-DEPENDENT PROTEASES IN BACTERIAL AND ANIMAL-CELLS [J].

GOLDBERG, AL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (1-2) :9-23

[10]

GOLDBERG AL, 1985, INTRACELLULAR PROTEI, P33

← 1 2 3 4 5 →