Global transcriptional response of Staphylococcus aureus to Rhein, a Natural Plant Product

被引：74

作者：

Yu, Lu ^{[2
]}

Xiang, Hua ^{[1
]}

Fan, Junwen ^{[1
]}

Wang, Dacheng ^{[1
]}

Yang, Feng ^{[1
]}

Guo, Na ^{[1
]}

Jin, Qi ^{[3
]}

Deng, Xuming ^{[1
,2
]}

机构：

[1] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China

[2] Jilin Univ, Inst Zoonoses, Changchun 130062, Peoples R China

[3] Chinese Acad Med Sci, Inst Pathogen Biol, Beijing 100730, Peoples R China

来源：

JOURNAL OF BIOTECHNOLOGY | 2008年 / 135卷 / 03期

关键词：

Staphylococcus aureus; Rhein; GeneChip; transcription;

D O I：

10.1016/j.jbiotec.2008.04.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Staphylococcus aureus (S. aureus) is one of the most important pathogens in humans and animals, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. Rhein (RH), a natural plant product, has been reported to have potential antimicrobial activity against S. aureus, but the response mechanisms of S. aureus to RH are still poorly understood. RH showed good in vitro antibacterial activity against all 21 tested S. aureus strains in this experiment. We performed commercial Affymetrix GeneChips(TM) to determine the overall transcriptional response of S. aureus ATCC25923 triggered by the treatment of subinhibitory concentrations of RH at one time point 45 min. A total of 88 genes were identified to be differentially regulated by RH. Of these, 28 transporter genes were differentially regulated by RH; RH stress elevated the transcription of genes (srtB and isdABCDEFGI) encoding iron-regulated surface determinants system and genes (nrdIEF and nrdDG) involved in ribonucleotide reductase systems; but RH repressed genes (pflAB, nirBDR, narGH, ldh1, COL-SA0660, COL-SA2363 and COL-SA2386) responsible for anaerobic respiration and fermentation. To our knowledge, this genome-wide transcriptomics approach revealed first insights into the response of S. aureus to RH challenge. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：304 / 308

页数：5

共 26 条

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