Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1:: MexCD-OprJ is an inducible pump

被引：81

作者：

Morita, Y ^{[1
]}

Komori, Y ^{[1
]}

Mima, T ^{[1
]}

Kuroda, T ^{[1
]}

Mizushima, T ^{[1
]}

Tsuchiya, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Pharmaceut Sci, Dept Microbiol, Okayama 7008530, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 202卷 / 01期

关键词：

mex multidrug efflux pump; a series of deletion mutants; drug specificity; MexCD-OprM; Pseudomonas aeruginosa;

D O I：

10.1016/S0378-1097(01)00314-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：139 / 143

页数：5

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