Comparison of normalization methods with microRNA microarray

被引：41

作者：

Hua, You-Jia ^{[1
,2
,3
]}

Tu, Kang ^{[1
,3
]}

Tang, Zhong-Yi ^{[1
,3
]}

Li, Yi-Xue ^{[1
]}

Mao, Hua-Sheng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Syst Biol, Ctr Funct Genom,Bioinformat Ctr, Shanghai 200031, Peoples R China

[2] Natl Engn Ctr Biochip, Shanghai 201203, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Shanghai 200031, Peoples R China

来源：

GENOMICS | 2008年 / 92卷 / 02期

关键词：

microRNA microarray; normalization; print-tip loess;

D O I：

10.1016/j.ygeno.2008.04.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

MicroRNAs (miRNAs) are a group of RNAs that play important roles in regulating gene expression and protein translation. In a previous study, we established an oligonucleotide microarray platform to detect miRNA expression. Because it contained only hundreds of probes, data normalization was difficult. In this study, the microarray data for eight miRNAs extracted from inflamed rat dorsal root ganglion (DRG) tissue were normalized using 15 methods and compared with the results of real-time polymerase chain reaction. It was found that the miRNA microarray data normalized by the print-tip loess method were the most consistent with results from real-time polymerase chain reaction. Moreover, the same pattern was also observed in 14 different types of rat tissue. This study compares a variety of normalization methods and will be helpful in the preprocessing of miRNA microarray data. Crown Copyright (c) 2008 Published by Elsevier Inc. All rights reserved.

引用

页码：122 / 128

页数：7

共 25 条

[1] The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans [J].