Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase

被引:19
作者
Breyer, MJ [1 ]
Thompson, NE [1 ]
Burgess, RR [1 ]
机构
[1] UNIV WISCONSIN, MCARDLE LAB CANC RES, MADISON, WI 53706 USA
关键词
D O I
10.1128/jb.179.4.1404-1408.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A highly cross-reactive monoclonal antibody (MAb), 2G10, was found to react in a conserved region of Escherichia coli RNA polymerase sigma(70). The epitope was localized to amino acids 470 to 486, which included part of conserved region 3.1. The epitope for MAb 3D3, a MAb which maps close to the 2G10 epitope,,vas also determined.
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页码:1404 / 1408
页数:5
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