Messenger RNAs encoding lipoprotein lipase, fatty acid synthase and hormone-sensitive lipase in the adipose tissue of underfed-refed ewes and cows

被引:36
作者
Bonnet, M
Faulconnier, Y
Flechet, J
Hocquette, JF
Leroux, C
Langin, D
Martin, P
Chilliard, Y [1 ]
机构
[1] INRA, Lab Sous Nutr Ruminants, F-63122 St Genes Champanelle, France
[2] INRA, Lab Croissance & Metab Herbivores, F-63122 St Genes Champanelle, France
[3] INRA, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France
[4] Hop Rangueil, Inst Louis Bugnard, INSERM, U317, F-31403 Toulouse 4, France
来源
REPRODUCTION NUTRITION DEVELOPMENT | 1998年 / 38卷 / 03期
关键词
ruminants; adipose tissue; enzyme activities; mRNAs; refeeding;
D O I
10.1051/rnd:19980310
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mechanisms involved in the nutritional regulation of genes encoding lipogenic (lipoprotein lipase (LPL) and fatty acid synthase (FAS)) and lipolytic (hormone-sensitive lipase (HSL)) enzymes were investigated by comparing the levels of the corresponding mRNAs in the adipose tissue (AT) of underfed or underfed-refed ewes and cows. Refeeding sharply increased LPL and FAS activities (19-25- and 6-8-fold, respectively) and moderately increased (2-4 fold) the activities of glucose-6-phosphate dehydrogenase (G6PDH), malic enzyme (ME) and glycerol-3-phosphate dehydrogenase (G3PDH). Northern blot analysis revealed three LPL transcripts and a single FAS transcript in cow and ewe AT. A single HSL mRNA was detected in cow AT and two transcripts in ewe AT. Refeeding sharply increased LPL and FAS mRNA levels, while restriction slightly increased (cows) or had no effect (ewes) on the HSL mRNA levels. This suggests that nutritional factors regulate sharply the expression of LPL and FAS genes by pretranslational mechanisms, but less clearly that of HSL gene. (C) Inra/Elsevier, Paris.
引用
收藏
页码:297 / 307
页数:11
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