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The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA

被引：26
作者：
Hines, JC
Ray, DS
机构：
[1] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA
来源：
MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1998年 / 94卷 / 01期
关键词：
kinetoplast; minicircle; trypanosome; histone-like; mitochondrial;
D O I：
10.1016/S0166-6851(98)00048-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Crithidia fasciculata KAP1 gene encodes a small basic protein (p21) associated with kinetoplast DNA. The p21 protein has a nine amino acid cleavable presequence closely related to those of several other proteins targeted to the kinetoplast and binds non-specifically to kinetoplast minicircle DNA. The p21 protein also has a calculated pI of 13 with two amino acids (lysine and alanine) accounting for more than 50% of the residues and with 25 out of 28 lysine residues contained in the C-terminal half of the protein. Immunolocalization of p21 shows that the protein is found exclusively in the kinetoplast with a localization distinctly different from the antipodal localization of kinetoplast DNA topoisomerase and DNA polymerase, The KAP1 gene is a single copy gene and the KAPI mRNA is present at a constant level throughout the cell cycle. This highly basic protein may play a role in the condensation or segregation of the kinetoplast DNA, (C) 1998 Elsevier Science B.V. All rights reserved.
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页码：41 / 52
页数：12
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