High-level secretion of a virally encoded anti-fungal toxin in transgenic tobacco plants

被引：20

作者：

Park, CM ^{[1
]}

Berry, JO ^{[1
]}

Bruenn, JA ^{[1
]}

机构：

[1] SUNY BUFFALO, DEPT BIOL SCI, BUFFALO, NY 14260 USA

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 30卷 / 02期

关键词：

anti-fungal killer toxin; double-stranded RNA; gene expression; transgenic plant; Ustilago maydis;

D O I：

10.1007/BF00020122

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The KP4 toxin expressed in this transgenic plant was of the same size and specificity as the authentic Ustilago KP4 toxin. The expression level was at least 500 times higher than that of the KP6 toxin expressed in plants. Transgenic crop plants producing the KP4 toxin could be rendered resistant to KP4-susceptible fungal pathogens.

引用

页码：359 / 366

页数：8

共 21 条

[1] TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION OF RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE GENE-EXPRESSION IN LIGHT-GROWN AND DARK-GROWN AMARANTH COTYLEDONS [J].