Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor

被引：25

作者：

Lukachova, LV ^{[1
]}

Karyakin, AA ^{[1
]}

Ivanova, YN ^{[1
]}

Karyakina, EE ^{[1
]}

Varfolomeyev, SD ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia

来源：

ANALYST | 1998年 / 123卷 / 10期

关键词：

D O I：

10.1039/a803312i

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water-organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5-50 mM.

引用

页码：1981 / 1985

页数：5

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