Solvatochromic studies on reversed phase liquid chromatographic phases .2. Characterization of stationary and mobile phases

被引：33

作者：

Lu, HY ^{[1
]}

Rutan, SC ^{[1
]}

机构：

[1] VIRGINIA COMMONWEALTH UNIV,DEPT CHEM,RICHMOND,VA 23284

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 08期

关键词：

D O I：

10.1021/ac9507810

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

UV-visible spectra of three solvatochromic dyes, 4,6-dichloro-2-[2-(1-methyl-4-pyridinio)vinyl]phenolate, N-methyl-2-nitroaniline, and 2-nitroaniline, are employed to characterize the octadecyl-derivatized silica (ODS2) stationary phase and the corresponding mobile phases used in reversed-phase liquid chromatography. The mobile phases studied are methanol-water, acetonitrile-water, and tetrahydrofuran-water, with composition ranges from 100% to 5% of organic modifier. Across the whole mobile phase composition range, the ODS2 stationary phase shows three distinct behaviors upon variation of the mobile phase composition, which are described as non-retentive, solvated, and collapsed.

引用

页码：1387 / 1393

页数：7

共 40 条

[1] HETEROGENEITY OF REVERSED-PHASE CHROMATOGRAPHIC SURFACES - QUENCHING OF SORBED PYRENE FLUORESCENCE [J].