Hematopoietic stem cells engraft in mice with absolute efficiency

被引：105

作者：

Benveniste, P ^{[1
]}

Cantin, C ^{[1
]}

Hyam, D ^{[1
]}

Iscove, NN ^{[1
]}

机构：

[1] Univ Toronto, Ontario Canc Inst, Dept Med Biophys, Toronto, ON M5G 2M9, Canada

来源：

NATURE IMMUNOLOGY | 2003年 / 4卷 / 07期

关键词：

D O I：

10.1038/ni940

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The engraftment of murine hematopoietic stem cells (HSCs) into irradiated mice is thought to be an inefficient process, but has yet to be measured directly. We used two independent strategies to test their engraftment efficiency: one measured competition of unpurified donor bone marrow cells with recipient cells in murine hosts and the other tracked the engraftment of one highly purified stem cell injected per recipient. The results showed that stem cells engrafted with near absolute efficiency. Thus, inefficient engraftment cannot explain the low frequency of permanent reconstitutions observed with pure HSC fractions and instead suggests most initially engrafted cells fail to sustain self-renewal.

引用

页码：708 / 713

页数：6

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