Identification of a novel aquaporin, AQP12, expressed in pancreatic acinar cells

被引：164

作者：

Itoh, T

Rai, T

Kuwahara, M

Ko, SBH

Uchida, S

Sasaki, S

Ishibashi, K

机构：

[1] Tokyo Med & Dent Univ, Grad Sch Med, Dept Nephrol, Bunkyo Ku, Tokyo 1138519, Japan

[2] Nagoya Univ, Grad Sch Med, Dept Med, Div Gastroenterol, Nagoya, Aichi 4668550, Japan

[3] China E Natl Hosp, Clin Res Ctr, Div Mol Biol Res, Chuo Ku, Chiba 2608712, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 03期

关键词：

aquaporin; pancreas; acinar cell; AQP; water channel;

D O I：

10.1016/j.bbrc.2005.03.046

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the aquaporin (AQP) water channel family are widely distributed in various tissues and contribute to the water permeability of epithelial and endothelial cells. Currently I I members of the AQP family (AQP0-10) have been reported in mammals. Here we report the identification of AQP12, which we found by performing a BLAST program search. Northern blot analysis revealed that AQP12 was specifically expressed in the pancreas. Further analysis by in situ hybridization and RT-PCR studies showed that AQP12 was selectively localized in the acinar cells of the pancreas. To analyze the cellular localization and function of AQP12, we expressed AQP12 in Xenopus oocytes and cultured mammalian cells. Immunocytochemistry revealed that AQP12 was not targeted to the plasma membrane. The selective localization of AQP12 in pancreatic acinar cells and possibly in the intracellular organelles suggests a role of AQP12 in digestive enzyme secretion such as maturation and exocytosis of secretory granules. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：832 / 838

页数：7

共 16 条

[1] Pathogenesis of nephrogenic diabetes insipidus by aquaporin-2 C-terminus mutations [J].