15N and 13C natural abundance of autotrophic and mycoheterotrophic orchids provides insight into nitrogen and carbon gain from fungal association

被引:253
作者
Gebauer, G [1 ]
Meyer, M [1 ]
机构
[1] Univ Bayreuth, Lehrstuhl Pflanzenokol, D-95440 Bayreuth, Germany
关键词
delta C-13; delta N-15; forest; grassland; myco-heterotrophy; mycorrhiza; orchids; stable isotopes;
D O I
10.1046/j.1469-8137.2003.00872.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Whereas mycorrhizal fungi are acknowledged to be the sources of nitrogen (N) and carbon (C) in achlorophyllous (myco-heterotrophic) orchids, the sources of these elements in autotrophic orchids are unknown. We have determined the stable isotope abundance of N and C to quantify their gain from different sources in these two functional groups and in non-orchids of distinctive mycorrhizal types. Leaves of each plant were collected from four forest and four grassland sites in Europe. The N and C isotope abundance, and total N concentrations of their tissues and of associated soils were determined. Myco-heterotrophic orchids were significantly more enriched in N-15 (epsilon(MHO-R) 11.5parts per thousand) and C-13 (epsilon(MHO-R) = 8.4%.) than co-occurring non-orchids. delta(15)N and delta(13)C signatures of autotrophic orchids ranged from values typical of non-orchids to those more representative of myco-heterotrophic orchids. Utilization of fungi-derived N and C probably explains the relative N-15 and C-13 enrichment in the myco-heterotrophs. A linear two-source isotopic mixing model was used to estimate N and C gain of autotrophic orchids from their fungal associates. Of the putatively autotrophic species, Cephalanthera damasonium obtained the most N and C by the fungal route, but several other species also fell into the partially myco-heterotrophic category.
引用
收藏
页码:209 / 223
页数:15
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