HPLC/UV analysis of proteins in dairy products using a hydrophobic interaction chromatographic column

被引:40
作者
Ferreira, IMPLVO [1 ]
Mendes, E [1 ]
Ferreira, MA [1 ]
机构
[1] Univ Porto, Fac Farm, Serv Bromatol, CEQUP, P-4050 Porto, Portugal
关键词
D O I
10.2116/analsci.17.499
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High-performance liquid chromatography using a Chrompack P-300-RP column containing a polystyrene-divinylbenzene copolymer-based packing was examined to analyze bovine milk protein components. The separation of major raw-milk proteins could be performed rapidly and reliably with this HPLC/UV method. The determinations were performed in the linear ranges of 0.01-2.0 mg/ml for alpha -lactalbumin, 0.04-2.5 mg/ml for caseins and 0.02-2.0 mg/ml for beta -lactoglobulin. The validity of the method was verified. Since the chromatographic column enabled the quantification of only "native" milk proteins, the extent of denaturation and loss of milk proteins could be examined. Thus, evaluation of heat-induced proteins denaturation was carried out in raw milk heated for 5 min at pre-determined temperatures.
引用
收藏
页码:499 / 501
页数:3
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