Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution

被引：39

作者：

Monzo, Alex ^{[1
]}

Bonn, Guenther K. ^{[1
]}

Guttman, Andras ^{[1
]}

机构：

[1] Univ Innsbruck, Inst Analyt Chem & Radiochem, Horvath Lab Bioseparat Sci, A-6020 Innsbruck, Austria

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 7-8期

关键词：

lectin; pseudo-lectin; affinity chromatography; electrophoresis;

D O I：

10.1007/s00216-007-1627-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce a novel combination of boronic acid affinity chromatography with lectin affinity chromatography, dubbed as boronic acid-lectin affinity chromatography (BLAC). Concanavalin A and wheat germ agglutinin lectins were mixed with the pesudo-lectin boronic acid to form the BLAC affinity column and their performance was evaluated with standard glycoproteins. Optimization of the binding and elution buffers for the BLAC system is described. The BLAC columns were employed to isolate glycoproteins of interest using both selective and/or combined elution.

引用

页码：2097 / 2102

页数：6

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