Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood

被引：412

作者：

Majeti, Ravindra ^{[1
,3
]}

Park, Christopher Y. ^{[2
,3
]}

Weissman, Irving L. ^{[2
,3
]}

机构：

[1] Stanford Univ, Dept Internal Med, Div Hematol, Palo Alto, CA 94304 USA

[2] Stanford Univ, Dept Pathol, Palo Alto, CA 94304 USA

[3] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Palo Alto, CA 94304 USA

来源：

CELL STEM CELL | 2007年 / 1卷 / 06期

关键词：

D O I：

10.1016/j.stem.2007.10.001

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mouse hematopoiesis is initiated by long-term hematopoietic stem cells (HSC) that differentiate into a series of multipotent progenitors that exhibit progressively diminished self-renewal ability. In human hematopoiesis, populations enriched for HSC activity have been identified, as have downstream lineage-committed progenitors, but multipotent progenitor activity has not been uniquely isolated. Previous reports indicate that human HSC are enriched in Lin-CD34+CD38- cord blood and bone marrow and express CD90. We demonstrate that the Lin-CD34+CD38- fraction of cord blood and bone marrow can be subdivided into three subpopulations: CD90+CD45RA-, CD90-CD45RA-, and CD90-CD45RA+. Utilizing in vivo transplantation studies and complementary in vitro assays, we demonstrate that the Lin-CD34+CD38-CD90+CD45RA- cord blood fraction contains HSC and isolate this activity to as few as 10 purified cells. Furthermore, we report the first prospective isolation of a population of candidate human multipotent progenitors, Lin-CD34+CD38-CD90-CD45RA cord blood.

引用

页码：635 / 645

页数：11

共 32 条

[1] ISOLATION OF A CANDIDATE HUMAN HEMATOPOIETIC STEM-CELL POPULATION [J].