Is the V3 loop involved in HIV binding to CD4?

被引：11

作者：

Dettin, M

Ferranti, P

Scarinci, C

Picariello, G

Di Bello, C

机构：

[1] Univ Padua, Dept Chem Proc Engn, I-35131 Padua, Italy

[2] CNR, Inst Food Sci & Technol, I-83100 Avellino, Italy

来源：

BIOCHEMISTRY | 2003年 / 42卷 / 30期

关键词：

D O I：

10.1021/bi0340696

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The entry of the human immunodeficiency virus into cells requires the interaction of the viral envelope glycoprotein gp120 with CD4 and a chemokine receptor. The gp120 binding site has been previously mapped to the Ig-CDR2-like region of CD4 first domain. A second area of this domain (Ig-CDR3-like region) is involved in gp120-CD4 interactions, but its gp120 counterpart remained so far unknown. Using a photoaffinity labeling experiment, we demonstrate that a peptide, mapping the (307-330)m region of HIV-MN-gp120 V3 loop, binds a sequence including a part of the Ig-CDR3-like region. These results may contribute to explain the complex mechanism of human immunodeficiency virus penetration, helping the development of new therapeutic agents.

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页码：9007 / 9012

页数：6

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