The re1A homolog of Mycobacterium smegmatis affects cell appearance, viability, and gene expression

被引：50

作者：

Dahl, JL

Arora, K

Boshoff, HI

Whiteford, DC

Pacheco, SA

Walsh, OJ

Lau-Bonilla, D

Davis, WB

Garza, AG

机构：

[1] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA

[2] NIAID, TB Sect, Rockville, MD USA

[3] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA

来源：

JOURNAL OF BACTERIOLOGY | 2005年 / 187卷 / 07期

关键词：

D O I：

10.1128/JB.187.7.2439-2447.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The modification of metabolic pathways to allow for a dormant lifestyle appears to be an important feature for the survival of pathogenic bacteria within their host. One regulatory mechanism for persistent Mycobacterium tuberculosis infections is the stringent response. In this study, we analyze the stringent response of a nonpathogenic, saprophytic mycobacterial species, Mycobacterium smegmatis. The use of M. smegmatis as a tool for studying the mycobacterial stringent response was demonstrated by measuring the expression of two M. tuberculosis genes, hspX and eis, in M. smegmatis in the presence and absence of rel(Msm). The stringent response plays a role in M. smegmatis cellular and colony formation that is suggestive of changes in the bacterial cell wall structure.

引用

收藏

页码：2439 / 2447

页数：9

相关论文

共 38 条

[1] Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling [J].

Betts, JC ;

Lukey, PT ;

Robb, LC ;

McAdam, RA ;

Duncan, K .

MOLECULAR MICROBIOLOGY, 2002, 43 (03) :717-731

[2] A RAPID AND GENTLE METHOD FOR THE ISOLATION OF GENOMIC DNA FROM MYCOBACTERIA [J].

BOSE, M ;

CHANDER, A ;

DAS, RH .

NUCLEIC ACIDS RESEARCH, 1993, 21 (10) :2529-2530

[3] Expression of Mycobacterium smegmatis pyrazinamidase in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides [J].

Boshoff, HIM ;

Mizrahi, V .

JOURNAL OF BACTERIOLOGY, 2000, 182 (19) :5479-5485

[4] DksA affects ppGpp induction of RpoS at a translational level [J].

Brown, L ;

Gentry, D ;

Elliott, T ;

Cashel, M .

JOURNAL OF BACTERIOLOGY, 2002, 184 (16) :4455-4465

[5] Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis -: Evidence that this lipid is involved in the cell wall permeability barrier [J].

Camacho, LR ;

Constant, P ;

Raynaud, C ;

Lanéelle, MA ;

Triccas, JA ;

Gicquel, B ;

Daffé, M ;

Guilhot, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (23) :19845-19854

[6]

Cashel M, 1996, ESCHERICHIA COLI SAL, P1458

[7] Mycobacterial stationary phase induced by low oxygen tension:: Cell wall thickening and localization of the 16-kilodalton α-crystallin homology [J].

Cunningham, AF ;

Spreadbury, CL .

JOURNAL OF BACTERIOLOGY, 1998, 180 (04) :801-808

[8] The role of RelMtb-mediated adaptation to stationary phase in long-term persistence of Mycobacterium tuberculosis in mice [J].

Dahl, JL ;

Kraus, CN ;

Boshoff, HIM ;

Doan, B ;

Foley, K ;

Avarbock, D ;

Kaplan, G ;

Mizrahi, V ;

Rubin, H ;

Barry, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (17) :10026-10031

[9] Electron microscopy analysis of Mycobacterium tuberculosis cell division [J].

Dahl, JL .

FEMS MICROBIOLOGY LETTERS, 2004, 240 (01) :15-20

[10] Subcellular localization of the intracellular survival-enhancing Eis protein of Mycobacterium tuberculosis [J].

Dahl, JL ;

Wei, J ;

Moulder, JW ;

Laal, S ;

Friedman, RL .

INFECTION AND IMMUNITY, 2001, 69 (07) :4295-4302

← 1 2 3 4 →