Homozygous deletions and chromosome amplifications in human lung carcinomas revealed by single nucleotide polymorphism array analysis

被引：260

作者：

Zhao, XJ

Weir, BA

LaFramboise, T

Lin, M

Beroukhim, R

Garraway, L

Beheshti, J

Lee, JC

Naoki, K

Richards, WG

Sugarbaker, D

Chen, F

Rubin, MA

Jänne, PA

Girard, L

Minna, J

Christiani, D

Li, C

Sellers, WR

Meyerson, M

机构：

[1] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA

[2] Dana Farber Canc Inst, Dept Biostat Sci, Boston, MA 02115 USA

[3] Dana Farber Canc Inst, Lowe Ctr Thorac Oncol, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Dept Pathol, Cambridge, MA 02138 USA

[5] Harvard Univ, Sch Med, Dept Med, Cambridge, MA 02138 USA

[6] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA

[7] Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA USA

[8] Brigham & Womens Hosp, Dept Pathol, Boston, MA USA

[9] Brigham & Womens Hosp, Dept Med, Boston, MA USA

[10] Brigham & Womens Hosp, Div Thorac Surg, Boston, MA USA

[11] Yokohama Municipal Citizens Hosp, Dept Resp Med, Yokohama, Kanagawa, Japan

[12] Univ Texas, SW Med Ctr, Dept Internal Med & Pharmacol, Dallas, TX USA

[13] MIT, Cambridge, MA USA

[14] Broad Inst, Cambridge, MA USA

来源：

CANCER RESEARCH | 2005年 / 65卷 / 13期

关键词：

D O I：

10.1158/0008-5472.CAN-04-4603

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Genome-wide copy number changes were analyzed in 70 primary human lung carcinoma specimens and 31 cell lines derived from human lung carcinomas, with high-density arrays representing similar to 115,000 single nucleotide polyrnorphism loci. In addition to previously characterized loci, two regions of homozygous deletion were found, one near the PTPRD locus on chromosome segment 9p23 in four samples representing both small cell lung carcinoma (SCLC) and non-small cell lung carcinoma (NSCLC) and the second on chromosome segment 3q25 in one sample each of NSCLC and SCLC. Highlevel amplifications were identified within chromosome segment 8q12-13 in two SCLC specimens, 12p11 in two NSCLC specimens and 22q11 in four NSCLC specimens. Systematic copy number analysis of tyrosine kinase genes identified highlevel amplification of EGFR in three NSCLC specimens, FGFR1 in two specimens and ERBB2 and MET in one specimen each. EGFR amplification was shown to be independent of kinase domain mutational status.

引用

页码：5561 / 5570

页数：10

共 56 条

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