Evaluation of a group-specific 16S ribosomal DNA-based PCR for detection of Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis in fresh and paraffin-embedded gastric biopsy specimens

被引:19
作者
De Groote, D
Haesebrouck, F
van Doorn, LJ
Vandamme, P
Ducatelle, R
机构
[1] State Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Avian Dis, B-9820 Merelbeke, Belgium
[2] State Univ Ghent, Fac Pharmaceut Sci, Microbiol Lab, B-9000 Ghent, Belgium
[3] Delft Diagnost Lab, NL-2600 GA Delft, Netherlands
关键词
D O I
10.1128/JCM.39.3.1197-1199.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new specific and sensitive 16S ribosomal DNA based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin embedded gastric biopsy specimens.
引用
收藏
页码:1197 / 1199
页数:3
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