Cutting edge: CTLs rapidly capture membrane fragments from target cells in a TCR signaling-dependent manner

被引:175
作者
Hudrisier, D
Riond, J
Mazarguil, H
Gairin, JE
Joly, E
机构
[1] CHU Purpan, INSERM, Unite 395, F-31024 Toulouse 03, France
[2] CHU Purpan, INSERM, Unite Mixte Rech 5089, F-31024 Toulouse, France
[3] CHU Purpan, INSERM, Unite Propre Rech 2163, F-31024 Toulouse 03, France
关键词
D O I
10.4049/jimmunol.166.6.3645
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Upon encounter of a CTL with a target cell carrying foreign Ags, the TCR internalizes with its ligand, the peptide-MHC class I complex. However, it is unclear how this can happen mechanistically because MHC molecules are anchored to the target cell's surface via a transmembrane domain. By using antigenic peptides and lipids that were fluorescently labeled, we found that CTLs promptly capture target cell membranes together with the antigenic peptide as well as various other surface proteins. This efficient and specific capture process requires sustained TCR signaling. Our observations indicate that this process allows efficient acquisition of the Ag by CTL, which may in turn regulate lymphocyte activation or elimination.
引用
收藏
页码:3645 / 3649
页数:5
相关论文
共 20 条
[1]  
Arnold PY, 1999, EUR J IMMUNOL, V29, P1363, DOI 10.1002/(SICI)1521-4141(199904)29:04<1363::AID-IMMU1363>3.0.CO
[2]  
2-0
[3]   THE BRIDE OF SEVENLESS AND SEVENLESS INTERACTION - INTERNALIZATION OF A TRANSMEMBRANE LIGAND [J].
CAGAN, RL ;
KRAMER, H ;
HART, AC ;
ZIPURSKY, SL .
CELL, 1992, 69 (03) :393-399
[4]   Critical role for CD8 in T cell receptor binding and activation by peptide/major or histocompatibility complex multimers [J].
Daniels, MA ;
Jameson, SC .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 191 (02) :335-345
[5]   The dynamics of T cell receptor signaling: Complex orchestration and the key roles of tempo and cooperation [J].
Germain, RN ;
Stefanova, I .
ANNUAL REVIEW OF IMMUNOLOGY, 1999, 17 :467-522
[6]   Fratricide among CD8+ T lymphocytes naturally infected with human T cell lymphotropic virus type I [J].
Hanon, E ;
Stinchcombe, JC ;
Saito, M ;
Asquith, BE ;
Taylor, GP ;
Tanaka, Y ;
Weber, JN ;
Griffiths, GM ;
Bangham, CRM .
IMMUNITY, 2000, 13 (05) :657-664
[7]   TCR-mediated internalization of peptide-MHC complexes acquired by T cells [J].
Huang, JF ;
Yang, Y ;
Sepulveda, H ;
Shi, WX ;
Hwang, I ;
Peterson, PA ;
Jackson, MR ;
Sprent, J ;
Cai, ZL .
SCIENCE, 1999, 286 (5441) :952-954
[8]   RELATIVE IMPLICATION OF PEPTIDE RESIDUES IN BINDING TO MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I H-2D(B) - APPLICATION TO THE DESIGN OF HIGH-AFFINITY, ALLELE-SPECIFIC PEPTIDES [J].
HUDRISIER, D ;
MAZARGUIL, H ;
OLDSTONE, MBA ;
GAIRIN, JE .
MOLECULAR IMMUNOLOGY, 1995, 32 (12) :895-907
[9]   Genetically encoded and post-translationally modified forms of a major histocompatibility complex class I-restricted antigen bearing a glycosylation motif are independently processed and co-presented to cytotoxic T lymphocytes [J].
Hudrisier, D ;
Riond, J ;
Mazarguil, H ;
Oldstone, MBA ;
Gairin, JE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (51) :36274-36280
[10]  
Hudrisier D, 1998, J IMMUNOL, V161, P553