Apoptosis-induced cleavage of keratin 15 and keratin 17 in a human breast epithelial cell line

被引：30

作者：

Badock, V

Steinhusen, U

Bommert, K

Wittmann-Liebold, B

Otto, A

机构：

[1] Max Delbruck Ctr Mol Med, Dept Med Oncol & Tumorimmunol, D-13092 Berlin, Germany

[2] Max Delbruck Ctr Mol Med, Dept Prot Chem, D-13092 Berlin, Germany

来源：

CELL DEATH AND DIFFERENTIATION | 2001年 / 8卷 / 03期

关键词：

apoptosis; epithelial cells; keratin; 15; 17; two-dimensional gel electrophoresis; caspases;

D O I：

10.1038/sj.cdd.4400812

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Keratin 15(K15)and keratin 17(K17)are intermediate filament (IF) type I proteins that are responsible for the mechanical integrity of epithelial cells. By analyzing the human breast epithelial cell line H184A1 before and after induction of apoptosis by high-resolution two dimensional gel electrophoresis (2-DE) we identified the caspase-mediated cleavage of keratins 15 and 17. After induction of apoptosis three fragments of both K15 and K17 could be observed by 2-DE. K15 and K17 proteolysis was observed during staurosporine-induced apoptosis and anoikis (anchorage-dependent apoptosis) as well and was shown to be caspase-dependent. By using mass spectrometry we could determine the caspase cleavage sites, one in K15 and two in K17, The sequence VEMD/A at the cleavage site located in the conserved linker region was found in K15 and K17, A further cleavage site was identified in the tail region of K17 with the recognition motif EVQD/G.

引用

页码：308 / 315

页数：8

共 32 条

[1] Identification of apoptosis-associated proteins in a human Burkitt lymphoma cell line -: Cleavage of heterogeneous nuclear ribonucleoprotein A1 by caspase 3 [J].