Fluorometric investigation of the interaction of bovine serum albumin with surfactants and 6-mercaptopurine

被引：132

作者：

Hu, YJ

Liu, Y ^{[1
]}

Jiang, W

Zhao, RM

Qu, SS

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Dept Chem, Wuhan 430072, Peoples R China

[2] Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China

[3] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2005年 / 80卷 / 03期

基金：

中国国家自然科学基金;

关键词：

6-mercaptopurine bovine serum albumin; fluorescence spectroscopy; UV-visible spectroscopy; ionic surfactant; fluorescence resonance energy transfer;

D O I：

10.1016/j.jphotobiol.2005.04.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence quenching in solutions of bovine serum albumin has been investigated in the presence of 6-mercaptopurine and ionic surfactants. Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of bovine serum albumin by 6-mercaptopurine was dynamic quenching mechanism. The Stern-Volmer quenching model has been successfully applied, and the activation energy of the interaction between 6-mercaptopurine and bovine serum albumin as much as 4.26 kJ mol(-1) was calculated. The distance r between donor (bovine serum albumin) and acceptor (6-mercaptopurine) was obtained according to fluorescence resonance energy transfer (FRET). The result of synchronous fluorescence spectra shows that the conformation of bovine serum albumin has been changed at the present of 6-mercaptopurine. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：235 / 242

页数：8

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