Presence of two different active nirS nitrite reductase genes in a denitrifying Thauera sp from a high-nitrate-removal-rate reactor

被引：91

作者：

Etchebehere, C

Tiedje, J

机构：

[1] Univ Republica, Fac Quim, Catedra Microbiol, Montevideo, Uruguay

[2] Univ Republica, Fac Sci, Dept Microbiol, Montevideo, Uruguay

[3] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 09期

关键词：

D O I：

10.1128/AEM.71.9.5642-5645.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nirS nitrite reductase genes were studied in two strains (strains 27 and 28) isolated from two denitrifying reactors and characterized as Thauera according to their 16S rRNA gene sequences. Strain 28 contains a single nirS sequence, which is related to the nirS of Thauera mechernichensis, and strain 27 contains two nirS sequences; one is similar to the nirS sequence from Thauera mechernichensis (gene 2), but the second one (gene 8) is from a separate clade with nirS from Pseudomonas stutzeri, Azoarcus species, Alcaligenes faecalis, and other Thauera species. Both genes were expressed, but gene 8 was constitutively expressed while gene 2 was positively regulated by nitrate.

引用

页码：5642 / 5645

页数：4

共 13 条

[1] Effect of soil ammonium concentration on N2O release and on the community structure of ammonia oxidizers and denitrifiers [J].