Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1

被引:40
作者
Liebertz, Daniel J. [1 ]
Lechner, Melissa G. [1 ]
Masood, Rizwan [2 ]
Sinha, Uttam K. [2 ]
Han, Jing [3 ]
Puri, Raj K. [3 ]
Correa, Adrian J. [1 ]
Epstein, Alan L. [1 ]
机构
[1] Univ So Calif, Keck Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
[2] Univ So Calif, Keck Sch Med, Dept Otolaryngol, Los Angeles, CA 90033 USA
[3] US FDA, Tumor Vaccines & Biotechnol Branch, Div Cellular & Gene Therapies, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA
关键词
GENE-EXPRESSION PROFILES; STEM-LIKE CELLS; CANCER; EXPANSION; BIOLOGY; PCR; HPV;
D O I
10.1186/1758-3284-2-5
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Head and neck squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy. Publically available cell lines are mostly of lingual origin, or have not been carefully characterized. Detailed characterization of novel HNSCC cell lines is needed in order to provide researchers a concrete keystone on which to build their investigations. Methods: The USC-HN1 cell line was established from a primary maxillary HNSCC biopsy explant in tissue culture. The immortalized cells were then further characterized by heterotransplantation in Nude mice; immunohistochemical staining for relevant HNSCC biomarkers; flow cytometry for surface markers; cytogenetic karyotypic analysis; human papillomavirus and Epstein-Barr virus screening; qRT-PCR for oncogene and cytokine analysis; investigation of activated, cleaved Notch1 levels; and detailed 35,000 gene microarray analysis. Results: Characterization experiments confirmed the human HNSCC origin of USC-HN1, including a phenotype similar to the original tumor. Viral screening revealed no HPV or EBV infection, while western blotting displayed significant upregulation of activated, cleaved Notch1. Conclusions: USC-HN1, a novel immortalized cell line has been derived from a maxillary HNSCC. Characterization studies have shown that the cell line is of HNSCC origin and displays many of the same markers previously reported in the literature. USC-HN1 is available for public research and will further the investigation of HNSCC and the development of new therapeutic modalities.
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页数:14
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共 24 条
  • [1] T regulatory type 1 cells in squamous cell carcinoma of the head and neck: Mechanisms of suppression and expansion in advanced disease
    Bergmann, Christoph
    Strauss, Laura
    Wang, Yun
    Szczepanski, Miroslaw J.
    Lang, Stephan
    Johnson, Jonas T.
    Whiteside, Theresa L.
    [J]. CLINICAL CANCER RESEARCH, 2008, 14 (12) : 3706 - 3715
  • [2] Burk RD, 2003, CANCER RES, V63, P7215
  • [3] Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma
    Chiou, Shih-Hwa
    Yu, Cheng-Chia
    Huang, Chi-Yang
    Lin, Shu-Chun
    Liu, Chung-Ji
    Tsai, Tung-Hu
    Chou, Shiu-Huey
    Chien, Chian-Shiu
    Ku, Hung-Hai
    Lo, Jeng-Fan
    [J]. CLINICAL CANCER RESEARCH, 2008, 14 (13) : 4085 - 4095
  • [4] qPrimerDepot: a primer database for quantitative real time PCR
    Cui, Wenwu
    Taub, Dennis D.
    Gardner, Kevin
    [J]. NUCLEIC ACIDS RESEARCH, 2007, 35 : D805 - D809
  • [5] Culliney B, 2008, ONCOLOGY-NY, V22, P1152
  • [6] Characterization of gene expression profiles of 3 different human oral squamous cell carcinoma cell lines with different invasion and metastatic capacities
    Erdem, N. Fazil
    Carlson, Eric R.
    Gerard, David A.
    [J]. JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2008, 66 (05) : 918 - 927
  • [7] Notch emerges as new cancer drug target
    Garber, Ken
    [J]. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2007, 99 (17) : 1284 - 1285
  • [8] Role of molecular markers and gene profiling in head and neck cancers
    Gold, Kathryn A.
    Kim, Edward S.
    [J]. CURRENT OPINION IN ONCOLOGY, 2009, 21 (03) : 206 - 211
  • [9] Identification of potential therapeutic targets in human head & neck squamous cell carcinoma
    Han, Jing
    Kioi, Mitomu
    Chu, Wei-Sing
    Kasperbauer, Jan L.
    Strome, Scott E.
    Puri, Raj K.
    [J]. HEAD & NECK ONCOLOGY, 2009, 1 : 27
  • [10] HEO DS, 1989, CANCER RES, V49, P5167