Transcriptional regulation of adrenomedullin in rat vascular smooth muscle cells

被引：13

作者：

Hattori, Y ^{[1
]}

Mimura, A

Akimoto, K

Kasai, K

机构：

[1] Dokkyo Univ, Sch Med, Dept Endocrinol, Mibu, Tochigi 3210293, Japan

[2] Dokkyo Univ, Sch Med, Mol & Cellular Biol Lab, Mibu, Tochigi 3210293, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1999年 / 147卷 / 1-2期

关键词：

adrenomedullin; promoter; vascular smooth muscle;

D O I：

10.1016/S0303-7207(98)00222-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Adrenomedullin (AM), a potent vasorelaxant peptide recently identified in extracts of pheochromocytoma, is actively secreted from vascular smooth muscle cells (VSMC). AM production in rat VSMC is potently stimulated by proinflammatory cytokines including TNF alpha, and glucocorticoids and many vasoactive substances have also been shown to alter AM synthesis. To study AM promoter function, we cloned the 5'-flanking region of the rat AM gene, and AM promoter function was assessed in rat VSMC stably transfected with a construct containing the cloned fragment. This 1.4 kb AM promoter region includes a number of putative transcriptional control elements. The promoter activity in VSMC was stimulated by TNF alpha and dexamethasone, and was suppressed by 8-bromo-cAMP and forskolin. These data indicate that transcriptional regulation is an important mechanism for AM production in VSMC. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：143 / 147

页数：5

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