Raman microspectroscopy:: A comparison of point, line, and wide-field imaging methodologies

被引:158
作者
Schlücker, S [1 ]
Schaeberle, MD [1 ]
Huffman, SW [1 ]
Levin, IW [1 ]
机构
[1] NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1021/ac034169h
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Three different Raman microspectroscopic imaging methodologies using a single experimental configuration are compared; namely, point and line mapping, as representatives of serial imaging approaches, and direct or wide-field Raman imaging employing liquid-crystalline tunable filters are surveyed. Raman imaging data acquired with equivalent low-power 514.5-nm laser excitation and a cooled CCD camera are analyzed with respect to acquisition times, image quality, spatial resolution, intensity profiles along spatial coordinates, and spectral signal-to-noise ratios (SNRs). Point and line mapping techniques provide similar SNRs and reconstructed Raman images at spatial resolutions of -1.1 mum. In contrast, higher spatial resolution is obtained by direct, global imaging (similar to313 nm), allowing subtle morphological features on test samples to be resolved.
引用
收藏
页码:4312 / 4318
页数:7
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