Uni-dimensional double development HPTLC-densitometry method for simultaneous analysis of mangiferin and lupeol content in mango (Mangifera indica) pulp and peel during storage

被引：33

作者：

Jyotshna ^{[1
]}

Srivastava, Pooja ^{[1
]}

Killadi, Bharti ^{[2
]}

Shanker, Karuna ^{[1
]}

机构：

[1] Cent Inst Med & Aromat Plants, CSIR, Dept Analyt Chem, Lucknow 226016, Uttar Pradesh, India

[2] Cent Inst Subtrop Hort, Postharvest Management Div, Lucknow, Uttar Pradesh, India

来源：

FOOD CHEMISTRY | 2015年 / 176卷

关键词：

Mangifera indica; Dashehati; Mangiferin; Lupeol; HPTLC-densitometry; Storage effect; REVERSE-PHASE HPLC; PART II; L; IDENTIFICATION; FRUIT; EXPRESSION; GLYCOSIDES; ALKALOIDS; FLAVONOL; FRACTION;

D O I：

10.1016/j.foodchem.2014.12.034

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Mango (Mangifera indica) fruit is one of the important commercial fruit crops of India. Similar to other tropical fruits it is also highly perishable in nature. During storage/ripening, changes in its physicochemical quality parameters viz, firmness, titrable acidity, total soluble solid content (TSSC), carotenoids content, and other biochemicals are inevitable. A uni-dimensional double-development high-performance thin-layer chromatography (UDDD-HPTLC) method was developed for the real-time monitoring of mangiferin and lupeol in mango pulp and peel during storage. The quantitative determination of both compounds of different classes was achieved by densitometric HPTLC method. Silica gel 60F(254) HPTLC plates and two solvent systems viz. toluene/EtOAC/MeOH and EtOAC/MeOH, respectively were used for optimum separation and selective evaluation. Densitometric quantitation of mangiferin was performed at 390 nm, while lupeol at 610 nm after post chromatographic derivatization. Validated method was used to real-time monitoring of mangiferin and lupeol content during storage in four Indian cultivars, e.g. Bombay green (Bgreen), Dashehari, Langra, and Chausa. Significant correlations (p < 0.05) between of acidity and TSSC with mangiferin and lupeol in pulp and peel during storage were also observed. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：91 / 98

页数：8

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