Peptide and protein building blocks for synthetic biology: From programming biomolecules to self-organized biomolecular systems

被引:174
作者
Bromley, Elizabeth H. C. [1 ]
Channon, Kevin [1 ]
Moutevelis, Efrosini [1 ]
Woolfson, Derek N. [1 ,2 ]
机构
[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England
[2] Univ Bristol, Dept Biochem, Bristol BS8 1TD, Avon, England
关键词
D O I
10.1021/cb700249v
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There are several approaches to creating synthetic-biological systems. Here, we describe a molecular-design approach. First, we lay out a possible synthetic-biology space, which we define with a plot of complexity of components versus divergence from nature. In this scheme, there are basic units, which range from natural amino acids to totally synthetic small molecules. These are linked together to form programmable tectons, for example, amphipathic alpha-helices. In turn, tectons can interact to give self-assembled units, which can combine and organize further to produce functional assemblies and systems. To illustrate one path through this vast landscape, we focus on protein engineering and design. We describe how, for certain protein-folding motifs, polypeptide chains can be instructed to fold. These folds can be combined to give structured complexes, and function can be incorporated through computational design. Finally, we describe how protein-based systems may be encapsulated to control and investigate their functions.
引用
收藏
页码:38 / 50
页数:13
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