Preparative purification of the major anti-inflammatory triterpenoid esters from marigold (Calendula officinalis)

被引:92
作者
Hamburger, M
Adler, S
Baumann, D
Förg, A
Weinreich, B
机构
[1] Univ Jena, Inst Pharm, D-07743 Jena, Germany
[2] Adalbert Raps Zentrum Arznell & Gewurzpflanzenfor, D-85350 Freising Weihenstephan, Germany
关键词
Calendula officinalis; supercritical fluid extraction; faradiol; low-pressure liquid chromatography; preparative HPLC;
D O I
10.1016/S0367-326X(03)00051-0
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A method for the efficient preparative purification of faradiol. 3-O-laurate, palmitate, and myristate, the major anti-inflammatory triterpenoid esters in the flower heads of the medicinal plant Calendula officinalis has been developed. Gram quantities of the individual compounds were obtained with 96 to 98% purity by a combination of supercritical. fluid extraction (SFE), normal-phase and reversed-phase column chromatography. During the work-up of the faradiol esters, accompanying minor compounds of the triterpene ester fraction were purified and identified by spectroscopic means as maniladiol 3-O-laurate and myristate. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:328 / 338
页数:11
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