A flow cytometry-based assay to assess RSV-specific neutralizing antibody is reproducible, efficient and accurate

被引：38

作者：

Chen, M. ^{[1
]}

Chang, J. S. ^{[2
]}

Nason, M. ^{[3
]}

Rangel, D. ^{[4
]}

Gall, J. G. ^{[4
]}

Graham, B. S. ^{[1
]}

Ledgerwood, J. E. ^{[1
]}

机构：

[1] NIAID, Viral Pathogenesis Lab, Vaccine Res Ctr, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA

[2] Kaohsiung Med Univ, Dept Renal Care, Coll Med, Kaohsiung 807, Taiwan

[3] NIAID, Biostat Res Branch, Div Clin Res, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA

[4] GenVec Inc, Gaithersburg, MD 20878 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2010年 / 362卷 / 1-2期

关键词：

RSV; Neutralizing antibody; Reporter virus; RESPIRATORY SYNCYTIAL VIRUS; HEALTHY-ADULTS; CLINICAL-TRIAL; INFECTION; CELLS;

D O I：

10.1016/j.jim.2010.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Respiratory syncytial virus (RSV) is an important cause of respiratory infection in people of all ages, and is the leading cause of hospitalization in infants. Although commercially available monoclonal antibody is available for passive prophylaxis of neonates at risk of severe disease, there is no available vaccine to prevent RSV. Measurement of neutralizing activity will be a key endpoint for vaccine evaluation. Assessment of neutralizing antibody against RSV has been limited to traditional plaque reduction, which is time-consuming and inherently operator dependent and highly variable. Here, we describe a flow cytometry-based RSV-specific neutralization assay which is more rapid than traditional methods, highly sensitive and highly reproducible. Published by Elsevier B.V.

引用

页码：180 / 184

页数：5

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