Cloning and expression of α-D-glucosidase and N-acetyl-β-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus)

被引：33

作者：

Hughes, CV

Malki, G

Loo, CY

Tanner, ACR

Ganeshkumar, N

机构：

[1] Boston Univ, Goldman Sch Dent Med, Dept Pediat Dent, Boston, MA 02118 USA

[2] Forsyth Inst, Boston, MA USA

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 2003年 / 18卷 / 05期

关键词：

Tannerella forsythensis; Bacteroides forsythus; glycosidases; glucosidase; glycosaminidase;

D O I：

10.1034/j.1399-302X.2003.00091.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Screening a genomic library of Tannerella forsythensis (Bacteroides forsythus), using synthetic substrates conjugated to a fluorogen, 4-methylumbelliferone identified two glycosidase genes, which encode alpha-D-glucosidase and N-acetyl-beta-D-glucosaminidase, respectively. The alpha-D-glucosidase has a M-r of 81,141 and is homologous to an alpha-D-glucosidase from Bacteroides thetaiotaomicron. The N-acetyl-beta-D-glucosaminidase has a M-r of 87,787 and is homologous to an N-acetyl-beta-D-glucosaminidase in Porphyromonas gingivalis W83.

引用

页码：309 / 312

页数：4

共 21 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2]

Ausubel F. M., 1999, SHORT PROTOCOLS MOL

[3] PROTEOGLYCANS OF THE PERIODONTIUM - STRUCTURE, ROLE AND FUNCTION [J].