Selective fluorescence quenching of 2,3-diazabicyclo[2.2.2]oct-2-ene by nucleotides

被引：45

作者：

Marquez, C

Pischel, U

Nau, WM

机构：

[1] Int Jacobs Univ Bremen, Sch Sci & Engn, D-28759 Bremen, Germany

[2] Univ Porto, Dept Quim, REQUIMTE, P-4169007 Oporto, Portugal

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 21期

关键词：

D O I：

10.1021/ol035454q

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] The fluorescence quenching of 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) by nucleotides has been studied. The quenching mechanism was analyzed on the basis of deuterium isotope effects, tendencies for exciplex formation, and the quenching efficiency in the presence of a molecular container (cucurbit[7]uril). Exciplex-induced quenching appears to prevail for adenosine, cytidine, and uridine, while hydrogen abstraction becomes competitive for thymidine and guanosine. Compared to other fluorescent probes, DBO responds very selectively to the type of nucleotide.

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页码：3911 / 3914

页数：4

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