Salicylic acid produced by isochorismate synthase and isochorismate pyruvate lyase in various parts of constitutive salicylic acid producing tobacco plants

被引：10

作者：

Nugroho, LH ^{[1
]}

Verberne, MC ^{[1
]}

Verpoorte, R ^{[1
]}

机构：

[1] Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Pharmacognosy, NL-2300 RA Leiden, Netherlands

来源：

PLANT SCIENCE | 2001年 / 161卷 / 05期

关键词：

Nicotiana tabacum L. cv Samsun NN; constitutive salicylic acid production; isochorismate synthase; isochorismate pyruvate lyase; salicylic acid;

D O I：

10.1016/S0168-9452(01)00486-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Salicylic acid production in roots, stems, young leaves, medium leaves and old leaves of constitutive salicylic acid producing (CSA) tobacco plants was tested. In protein extracts of CSA-tobacco plants, the formation of isochorismate and of salicylic acid (SA) were measured to determine isochorismate synthase (ICS) and isochorismate pyruvate lyase (IPL) activity. The highest production of isochorismate and SA in vitro were produced by protein extracts of the young leaves of CSA-tobacco plants. The isochorismate and SA productions in vitro by protein extracts and the accumulation of SA and SA-glucoside in CSA-tobacco plants varied among the tested lines and within one line. The accumulation of SA in vivo is determined by the activity of IPL. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：911 / 915

页数：5

共 17 条

[1] Free and conjugated benzoic acid in tobacco plants and cell cultures. induced accumulation upon elicitation of defense responses and role as salicylic acid precursors [J].