The key step in chlorophyll breakdown in higher plants -: Cleavage of pheophorbide α macrocycle by a monooxygenase

被引:129
作者
Hörtensteiner, S
Wüthrich, KL
Matile, P
Ongania, KH
Kräutler, B
机构
[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria
[2] Univ Zurich, Dept Plant Biol, CH-8008 Zurich, Switzerland
关键词
D O I
10.1074/jbc.273.25.15335
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chlorophyll breakdown in green plants is a longstanding biological enigma. Recent work has shown that pheophorbide a (Pheide a) derived from chlorophyll (Chl) is converted oxygenolytically into a primary fluorescent catabolite (pFCC-1) via a red Chl catabolite (RCC) intermediate. RCC, the product of the ring cleavage reaction catalyzed by Pheide a oxygenase, which is suggested to be the key enzyme in Chi breakdown in green plants, is converted into pFCC-1 by a reductase. In the present study, an in vitro assay comprising O-18(2) Pheide a oxygenase and RCC reductase yielded labeled pFCC-1. Fast atom bombardment-mass spectrometric analysis of the purified pFCC-1 product revealed that only one of the two oxygen atoms newly introduced into Pheide a in the course of the cleavage reaction is derived from molecular oxygen. Analysis of the fragment ions located the oxygen atom derived from molecular oxygen on the formyl group of pyrrole B, This finding demonstrates that the cleavage of Pheide a in vascular plants is catalyzed by a monooxygenase. Chlorophyll breakdown is therefore indicated to be mechanistically related in higher plants and in the green alga Chlorella protothecoides.
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页码:15335 / 15339
页数:5
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