Inhibitory effects of 4-vinylbenzaldehyde and 4-vinylbenzoic acid on the activity of mushroom tyrosinase

被引：21

作者：

Song, KK ^{[1
]}

Chen, QX ^{[1
]}

Wang, Q ^{[1
]}

Qiu, L ^{[1
]}

Huang, H ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, Minist Educ Cell Biol & Tumor Cell Engn, Key Lab, Xiamen 361005, Peoples R China

来源：

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2005年 / 20卷 / 03期

关键词：

tyrosinase; monophenolase activity; diphenolase activity; 4-vinylbenzaldehyde; 4-vinylbenzoic acid; inhibition kinetics;

D O I：

10.1080/14756360400026238

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosinase (EC 1.14.18.1) catalyzes both the hydroxylation of ryrosine into o-diphenols and the oxidation of o-diphenols into o-quinones which form brown or black pigments. Here, the inhibitory effects of 4-vinylbenzaldehyde and 4-vinylbenzoic acid on the activity of mushroom tyrosinase have been investigated. The results showed that both 4-vinylbenzaldehyde and 4-vinylbenzoic acid could inhibit both monophenolase activity and diphenolase activity of the enzyme. For the monophenolase activity, 4-vinylbenzoic acid could lengthen the lag time, but 4-vinylbenzaldehyde could not. Both 4-vinylbenzaldehyde and 4-vinylbenzoic acid decreased the steady-state activity, and the IC50 values were estimated as 93 mu M and 3.0 mM for monophenolase activity, respectively. For the diphenolase activity, the inhibitory capacity of 4-vinylbenzaldehyde was stronger than that of 4-vinylbenzoic acid, and the IC50 values were estimated as 23 mu M and 0.33 mM, respectively. Kinetic analyses showed that inhibition by both compounds was reversible and their mechanisms were mixed-II type; their inhibition constants were also determined and compared.

引用

页码：239 / 243

页数：5

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