Metabolite profiling on apple volatile content based on solid phase microextraction and gas-chromatography time of flight mass spectrometry

被引：81

作者：

Aprea, Eugenio ^{[1
]}

Gika, Helen ^{[1
]}

Carlin, Silvia ^{[1
]}

Theodoridis, Georgios ^{[1
]}

Vrhovsek, Urska ^{[1
]}

Mattivi, Fulvio ^{[1
]}

机构：

[1] IASMA Res & Innovat Ctr, Fdn Edmund Mach, Food Qual & Nutr Area, I-38010 San Michele All Adige, TN, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 28期

关键词：

SPME; GC-TOF-MS; Apple (Malus domestica); Metabolite profiling; Metabolomics; RASPBERRY CULTIVARS; PTR-MS; BIOSYNTHESIS; SPME; IDENTIFICATION; REVEALS; ESTERS;

D O I：

10.1016/j.chroma.2011.05.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A heads pace SPME GC-TOF-MS method was developed for the acquisition of metabolite profiles of apple volatiles. As a first step, an experimental design was applied to find out the most appropriate conditions for the extraction of apple volatile compounds by SPME. The selected SPME method was applied in profiling of four different apple varieties by GC-EI-TOF-MS. Full scan GC-MS data were processed by MarkerLynx software for peak picking, normalisation, alignment and feature extraction. Advanced chemometric/statistical techniques (PCA and PLS-DA) were used to explore data and extract useful information. Characteristic markers of each variety were successively identified using the NIST library thus providing useful information for variety classification. The developed HS-SPME sampling method is fully automated and proved useful in obtaining the fingerprint of the volatile content of the fruit. The described analytical protocol can aid in further studies of the apple metabolome. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：4517 / 4524

页数：8

共 39 条

[1] Characterization of 14 Raspberry Cultivars by Solid-Phase Microextraction and Relationship with Gray Mold Susceptibility [J].