A fluorescently labeled, hyperbranched polymer synthesized from DE-ATRP for the detection of DNA hybridization

被引：13

作者：

Aied, Ahmed ^{[2
]}

Glynn, Barry ^{[3
]}

Cao, Hongliang ^{[2
]}

Zheng, Yu ^{[2
]}

Tai, Hongyun ^{[1
]}

Pandit, Abhay ^{[2
]}

Wang, Wenxin ^{[2
]}

机构：

[1] Bangor Univ, Sch Chem, Bangor, Gwynedd, Wales

[2] Natl Univ Ireland, Network Excellence Funct Biomat, Galway, Ireland

[3] Natl Univ Ireland, Mol Diagnost Res Grp, Galway, Ireland

来源：

POLYMER CHEMISTRY | 2012年 / 3卷 / 02期

基金：

爱尔兰科学基金会; 英国工程与自然科学研究理事会;

关键词：

CONJUGATED POLYMERS; MICRORNAS;

D O I：

10.1039/c2py00516f

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The early detection of oligonucleotide biomarkers of disease, such as microRNAs, has been established as a fundamental factor in cancer diagnosis. As the levels of these small molecules (microRNAs) in blood have recently been found to be significantly affected in cancer patients, they offer a means of early stage detection of cancer. Towards the goal of creating a novel method of DNA hybridization detection, we report the detection of specific sequences of small oligonucleotides in a model experiment carried out in serum. The results shown here display the versatility of the DE-ATRP method in synthesizing a specific polymer structure capable of changing its physical properties in the presence of double stranded DNA. The polymer was labeled and used to detect single-stranded DNA in serum successfully.

引用

页码：332 / 334

页数：3

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