Expression and purification of Src I from sea anemone Sagartia rosea as a recombinant non-fusion protein

被引：7

作者：

Jiang, XY

Peng, LS

Yang, WL

Tang, XJ

Liu, W

Xu, AL ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Coll Agr & Life Sci, Dept Biochem, Open Lab Marine Funct Genom State High Tech Dev, Guangzhou 510275, Peoples R China

[2] Guangdong Provincial Ctr Occupat Dis Prevent & Tr, Dept Toxicol, Guangzhou 510300, Peoples R China

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2003年 / 32卷 / 01期

关键词：

D O I：

10.1016/S1046-5928(03)00229-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA coding for an acidic actinoporin, Sagartia rosea cytolysin I (Src 1), has been isolated, cloned into pGEM-T Easy Vector, and sequenced. The region encoding matured Src I was also cloned into prokaryotic expression vector pBV220 and expressed in Escherichia coli as a non-fusion protein in the form of inclusion body. Through washing and denaturation-renaturation step, the recombinant Src I was purified by Q Sepharose Fast Flow ion exchange chromatography and Phenyl Sepharose hydrophobic interaction chromatography. The two-step purification of Src I was effective, simple, and suitable for isolating large amount of high purity (above 95%) recombinant Src 1. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：161 / 166

页数：6

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