Genetic control of rebaudioside A and C concentration in leaves of the sweet herb, Stevia rebaudiana

被引:26
作者
Brandle, J [1 ]
机构
[1] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada
关键词
diterpene glycoside; genetic analysis; glycosylation; biosynthetic model;
D O I
10.4141/P98-048
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Stevia rebaudiana Bertoni produces steviol glycoside sweeteners in its leaves that are up to 240 times sweeter than sugar. Understanding the genetic basis of glycoside proportions will aid in their manipulation through plant breeding. The experiments conducted in this study were focused on the genetic control of the proportions of two of those glycosides, rebaudioside A and rebaudioside C. The study was conducted using F(2) population from crosses between two sets of parents with divergent glycoside profiles. Segregation in the first set of F(2)s showed that the presence/absence of rebaudioside A is controlled by a single dominant gene, but that the actual proportions of rebaudioside A may be controlled by multiple loci or alleles. In a second cross, proportions of rebaudioside A and rebaudioside C were found to co-segregate and were shown to be controlled by a single additive gene. This result suggests that both rebaudioside A and C are synthesized by the same enzyme. The results were used to propose a model for glycosylation of steviol glycosides.
引用
收藏
页码:85 / 92
页数:8
相关论文
共 11 条
[1]   Stevia rebaudiana:: Its agricultural, biological, and chemical properties [J].
Brandle, JE ;
Starratt, AN ;
Gijzen, M .
CANADIAN JOURNAL OF PLANT SCIENCE, 1998, 78 (04) :527-536
[2]   HERITABILITY FOR YIELD, LEAF-STEM RATIO AND STEVIOSIDE CONTENT ESTIMATED FROM A LANDRACE CULTIVAR OF STEVIA-REBAUDIANA [J].
BRANDLE, JE ;
ROSA, N .
CANADIAN JOURNAL OF PLANT SCIENCE, 1992, 72 (04) :1263-1266
[3]  
CROTEAU R, 1994, RECENT ADV PHYTOCHEM, V28, P193
[4]  
Lee J. I., 1982, Research Reports, Office of Rural Development, S. Korea, Crop, V24, P186
[5]   TERPENOID METABOLISM [J].
MCGARVEY, DJ ;
CROTEAU, R .
PLANT CELL, 1995, 7 (07) :1015-1026
[6]  
MORITA T, 1987, Patent No. 96025
[7]  
SHAW DV, 1982, SILVA FENNICA, V16, P235
[8]   GLUCOSYLATION OF STEVIOL AND STEVIOL-GLUCOSIDES IN EXTRACTS FROM STEVIA-REBAUDIANA BERTONI [J].
SHIBATA, H ;
SONOKE, S ;
OCHIAI, H ;
NISHIHASHI, H ;
YAMADA, M .
PLANT PHYSIOLOGY, 1991, 95 (01) :152-156
[9]   STEVIOL AND STEVIOL-GLYCOSIDE - GLUCOSYLTRANSFERASE ACTIVITIES IN STEVIA-REBAUDIANA BERTONI - PURIFICATION AND PARTIAL CHARACTERIZATION [J].
SHIBATA, H ;
SAWA, Y ;
OKA, T ;
SONOKE, S ;
KIM, KK ;
YOSHIOKA, M .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 321 (02) :390-396
[10]  
STEEL R.G.D., 1980, PRINCIPLES PROCEDURE