Antiangiogenic activity of restin, NC10 domain of human collagen XV: Comparison to endostatin

被引：214

作者：

Ramchandran, R

Dhanabal, M

Volk, R

Waterman, MJF

Segal, M

Lu, H

Knebelmann, B

Sukhatme, VP

机构：

[1] Beth Israel Deaconess Med Ctr, Dept Med, Div Renal, Boston, MA 02215 USA

[2] Beth Israel Deaconess Med Ctr, Dept Med, Div Cardiol, Boston, MA 02215 USA

[3] Harvard Univ, Sch Med, Boston, MA 02215 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 03期

关键词：

D O I：

10.1006/bbrc.1999.0248

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eased on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic PET expression system. We have shown that restin inhibits the migration of endothelial cells in vitro but has no effect on the proliferation of these cells. A polyclonal antibody raised against endostatin cross-reacted with restin. Systemic administration of restin suppressed the growth of tumors in a xenograft renal carcinoma model, (C) 1999 Academic Press.

引用

页码：735 / 739

页数：5

共 13 条

[1] HUMAN INTERFERON-INDUCIBLE PROTEIN-10 IS A POTENT INHIBITOR OF ANGIOGENESIS IN-VIVO [J].