Interrupted catalytic domain structures in xylanases from two distantly related strains of Prevotella ruminicola

被引：30

作者：

Flint, HJ

Whitehead, TR

Martin, JC

Gasparic, A

机构：

[1] USDA ARS, NATL CTR AGR UTILIZAT RES, PEORIA, IL 61604 USA

[2] UNIV LJUBLJANA, BIOTECH FAC, DOMZALE 61230, LJUBLJANA, SLOVENIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1997年 / 1337卷 / 02期

关键词：

xylanase; rumen; domain structure; interrupting sequence; (P-ruminicola);

D O I：

10.1016/S0167-4838(96)00213-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two xylanases from the rumen anaerobic bacterium Prevotella ruminicola were found to possess highly unusual structures in which family 10 catalytic domains are interrupted by unrelated sequences. XynC from P. ruminicola B(1)4 carries a 160 amino-acid insertion, while a P. ruminicola D31d xylanase carries an unrelated region of 280 amino acids, containing an imperfect 130 amino-acid duplication. Both regions of family 10 similarity were shown to be essential for activity of the D31d enzyme.

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收藏

页码：161 / 165

页数：5

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