Outcome of laser-assisted polar body biopsy and aneuploidy testing

被引:49
作者
Montag, M [1 ]
van der Ven, K [1 ]
Dorn, C [1 ]
van der Ven, H [1 ]
机构
[1] Univ Clin Bonn, Dept Gynecol Endocrinol & Reprod Med, Bonn, Germany
关键词
aneuploidy; assisted reproduction; FISH; laser biopsy; maternal age; polar body analysis;
D O I
10.1016/S1472-6483(10)61278-3
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Polar body biopsy and subsequent fluorescence in-situ hybridization (FISH) analysis allows detection of maternally derived chromosomal aneuploidies in human oocytes during IVF treatment. The development of a diode laser technique for die, partial opening of the zona pellucida has stimulated the use of this technique to assist polar body biopsy. Laser-assisted polar body biopsy was performed in 140 IVF cycles from patients of advanced maternal age (greater than or equal to35 years). A total of 921 oocytes were treated by a laser for partial zona opening and polar body removal. FISH was performed for chromosomes 13, 16, 18, 21 and 22 and results were available for 903 oocytes (98%). In all, 443 oocytes (49.1%) were euploid and of these, 293 were fertilized. A total of 214 embryos were transferred in 120 embryo transfer cycles (1.78 per embryo transfer) resulting in 27 clinical pregnancies (22.5% per embryo transfer) with an implantation rate of 15.4%. Subsequently, five women aborted (18.5%) and 24 healthy children were born from the remaining 22 pregnancies, which gives a take home baby rate of 183% per transfer cycle. It is concluded that polar body biopsy using a diode laser system is as efficient as standard polar body biopsy using zona drilling.
引用
收藏
页码:425 / 429
页数:5
相关论文
共 19 条
[1]   Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching [J].
Cieslak, J ;
Ivakhnenko, V ;
Wolf, G ;
Sheleg, S ;
Verlinsky, Y .
FERTILITY AND STERILITY, 1999, 71 (02) :308-313
[2]  
GERMAN IVF, 2001, ARZTEKAMMER BAD SEGE
[3]   Preimplantation diagnosis for aneuploidies in patients undergoing in vitro fertilization with a poor prognosis:: identification of the categories for which it should be proposed [J].
Gianaroli, L ;
Magli, MC ;
Ferraretti, AP ;
Munné, S .
FERTILITY AND STERILITY, 1999, 72 (05) :837-844
[4]   A follow-up study of children born after diode laser assisted hatching [J].
Kanyó, K ;
Konc, J .
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2003, 110 (02) :176-180
[5]  
Kuliev Anver, 2003, Reprod Biomed Online, V6, P54
[6]  
Montag M., 1997, Journal of Assisted Reproduction and Genetics, V14, P455
[7]   Success of intracytoplasmic sperm injection in couples with male and/or female chromosome aberrations [J].
Montag, M ;
van der Ven, K ;
Ved, S ;
Schmutzler, A ;
Prietl, G ;
Krebs, D ;
Peschka, B ;
Schwanitz, G ;
Albers, P ;
Haidl, G ;
van der Ven, H .
HUMAN REPRODUCTION, 1997, 12 (12) :2635-2640
[8]   Embryo transfer on day 3 using low versus high fluid volume [J].
Montag, M ;
Kupka, M ;
van der Ven, K ;
van der Ven, H .
EUROPEAN JOURNAL OF OBSTETRICS GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 2002, 102 (01) :57-60
[9]   Laser-assisted microdissection of the zona pellucida facilitates polar body biopsy [J].
Montag, M ;
van der Ven, K ;
Delacrétaz, G ;
Rink, K ;
van der Ven, H .
FERTILITY AND STERILITY, 1998, 69 (03) :539-542
[10]  
Montag M, 1999, CROAT MED J, V40, P398