Cell surface expression of 5-hydroxytryptamine type 3 receptors is promoted by RIC-3

被引:59
作者
Cheng, AX [1 ]
McDonald, NA [1 ]
Connolly, CN [1 ]
机构
[1] Univ Dundee, Ninewells Med Sch, Dept Pharmacol & Neurosci, Dundee DD1 9SY, Scotland
关键词
D O I
10.1074/jbc.M414341200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RIC-3 has been identified as a molecule essential for the recruitment of functional nicotinic acetylcholine receptors composed of a7, but it exhibits inhibitory effects on a4b2 or a3b4 receptors. In this study, we investigated the role of RIC-3 in the recruitment of 5-hydroxytryptamine type 3A (5-HT3A3A3A) receptors to the cell surface. Although RIC-3 is not essential for the surface transport of 5-HT3A receptors, we found that its presence enhances both receptor transport and function in a concentration-dependent manner. RIC-3 is localized to the endoplasmic reticulum, as evidenced by co-localization with the chaperone molecule, binding protein (BiP). RIC-3 is not detected at significant levels on the cell surface when expressed alone or in the presence of 5-HT. RIC-3 and 5-HT (show a low level interaction that is transient (< 4 h). That RIC-3 can interact with an endoplasmic reticulum-retained 5-HT)(3A) (construct, combined with the transient interaction observed and lack of significant surface-expressed RIC)(, suggests that RIC)(-3)(-3) (may play a role in 5-HT)(3A) receptor folding, assembly, or transport to the cell surface.
引用
收藏
页码:22502 / 22507
页数:6
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