Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection

被引：33

作者：

Faux, Cassandra E. ^{[2
]}

Arden, Katherine E. ^{[2
]}

Lambert, Stephen B. ^{[2
]}

Nissen, Michael D. ^{[2
]}

Nolan, Terry M. ^{[3
]}

Chang, Anne B.

Sloots, Theo P. ^{[2
]}

Mackay, Ian M. ^{[1
,2
]}

机构：

[1] Royal Childrens Hosp, Queensland Childrens Med Res Inst, Queensland Paediat Infect Dis Lab, Sir Albert Sakzewski Virus Res Ctr, Brisbane, Qld, Australia

[2] Univ Queensland, Brisbane, Qld, Australia

[3] Univ Melbourne, Melbourne, Vic, Australia

来源：

EMERGING INFECTIOUS DISEASES | 2011年 / 17卷 / 02期

基金：

英国医学研究理事会;

关键词：

POLYMERASE CHAIN-REACTION; RESPIRATORY VIRUSES; CLINICAL SPECIMENS; NUCLEIC-ACIDS; RT-PCR; CHILDREN; AMPLIFICATION; ASSAY;

D O I：

10.3201/eid1702.101123

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV) specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

引用

页码：296 / 298

页数：3

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