Molecular detection and quantitation of the red tide dinoflagellate Karenia brevis in the marine environment

被引:54
作者
Gray, M [1 ]
Wawrik, B [1 ]
Paul, J [1 ]
Casper, E [1 ]
机构
[1] Univ S Florida, Coll Marine Sci, St Petersburg, FL 33701 USA
关键词
D O I
10.1128/AEM.69.9.5726-5730.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A real-time reverse transcription-PCR method targeting the rbcL gene was developed for the detection and quantitation of the Florida red tide organism, Karenia brevis. The assay was sensitive to less than 1 cell per reaction, did not detect rbcL from 38 nontarget taxa, and accurately quantitated K. brevis organisms in red tide samples from around Florida. These studies have resulted in a sensitive and specific method for K. brevis detection in the marine environment.
引用
收藏
页码:5726 / 5730
页数:5
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